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Kevin Myles

Myles, Kevin
Kevin Myles
Associate Professor
Office:
Heep Center 329B
Email:
Phone:
979-458-3110
Undergraduate Education
B.S. Microbiology - Colorado State University - 1997
Graduate Education
Ph.D. Microbiology - Colorado State University - 2003

Publications:

Basu S, Aryan A, Overcash JM, Samuel GH, Anderson MA, Dahlem TJ, Myles KM, Adelman ZN (2015) Silencing of end-joining repair for efficient site-specific gene insertion after TALEN/CRISPR mutagenesis in Aedes aegypti. Proc. Natl. Acad. Sci. U.S.A. 112(13): 4038-43

Haac ME, Anderson MA, Eggleston H, Myles KM, Adelman ZN (2015) The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes. Nucleic Acids Res. 43(7): 3688-700

Overcash JM, Aryan A, Myles KM, Adelman ZN (2015) Understanding the DNA damage response in order to achieve desired gene editing outcomes in mosquitoes. Chromosome Res. 23(1): 31-42

O’Neal ST, Samuel GH, Adelman ZN, Myles KM (2014) Mosquito-borne viruses and suppressors of invertebrate antiviral RNA silencing. Viruses 6(11): 4314-31

Liu M, Adelman ZN, Myles KM, Zhang L (2014) A transcriptome post-scaffolding method for assembling high quality contigs. Computational Biology Journal Article ID 961823, 4 pages

Allen WJ, Wiley MR, Myles KM, Adelman ZN, Bevan DR (2014) Steered molecular dynamics identifies critical residues of the Nodomura virus suppressor of RNAi. J. Mol Model 20(3): 2092

Aryan A, Myles KM, Adelman ZN (2014) Targeted genome editing in Aedes aegypti using TALENs. Methods 69(1): 38-45

Adelman ZN, Miller DM, Myles KM (2013) Bed bugs and infectious disease: a case for the arboviruses. PLoS Pathog 9(8):e1003462.

Aryan A, Anderson MA, Myles KM, and Adelman ZN. (2013) TALEN-based gene disruption in the dengue vector Aedes aegypti. PLoS ONE. 8: e60082

Lucas KJ, Myles KM, and Raikhel AS. (2013) Small RNAs: a new frontier in mosquito biology. Trends Parasitol. 29: 295-303

Adelman ZN, Anderson MA, Wiley MR, Murreddu MG, Samuel GH, Morazzani EM, and Myles KM. (2013) Cooler temperatures destabilize RNA interference and increase susceptibility of disease vector mosquitoes to viral infection. PLoS Negl. Trop. Dis. 7: e2239

Aryan A, Anderson MA, Myles KM, and Adelman ZN. (2013) Germline excision of transgenes in Aedes aegypti by homing endonucleases. Sci. Rep. 3: 1603

Morazzani EM, Wiley MR, Murreddu MG, Adelman ZN, Myles KM (2012) Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma. PLoS Pathog 8: 1-11 e1002470

Adelman ZN, Anderson MAE, Liu M, Zhang L, and Myles KM (2012) Sindbis virus induces the production of a novel class of endogenous siRNAs in Aedes aegypti mosquitoes Insect Mol Bio 21: 357-68

Carpenetti TL, Aryan A, Myles KM, Adelman ZN (2012) Robust heat-inducible gene expression by two endogenous hsp70-derived promoters in transgenic Aedes aegypti. Insect Mol Bio 21: 97-106

Wiley, M.R., Roberts, L., Adelman, Z.N., and Myles, K.M. (2010) Double subgenomic alphaviruses expressing multiple fluorescent proteins using a Rhopalosiphum padi virus internal ribosome entry site element. PLoS ONE 5: 1-8 e13924.

Anderson, M.A.E., Gross, T. L., Myles, K. M, and Adelman, Z.N. (2010) Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti. Insect Mol Bio 19: 441-449.

Plaskon NE, Adelman ZN, Myles KM. (2009). Accurate Strand-Specific Quantification of Viral RNA. PLoS ONE. 4: 1-8 e7468

Myles KM, Morazzani EM, and Adelman ZN. (2009). Origins of alphavirus-derived small RNAs in mosquitoes. RNA Biology. 6: 1-5.

Gross TL, Myles KM, and Adelman ZN. (2009). Identification and characterization of heat shock 70 genes in Aedes aegypti (Diptera: Culicidae). J. Med. Entomol. 46:496-504

Myles KM, Wiley MR, Morazzani EM, and Adelman ZN. (2008). Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes. Proc. Natl. Acad. Sci. U. S. A. 105: 19938-19943.

Adelman ZN, Anderson MAE, Morazzani EM, and Myles KM. (2008). A transgenic sensor strain for monitoring the RNAi pathway in the yellow fever mosquito, Aedes aegypti. Insect Biochem. Mol. Biol. 38:705-713.

Myles KM, Kelly CLH, Ledermann JP, Powers AM. (2006). Effects of an opal termination codon preceding the nsP4 gene sequence in the o”nyong-nyong virus genome on Anopheles gambiae infectivity. J. Virol. 80:4992-4997

Brault AC, Foy BD, Myles KM, Higgs S, Weaver SC, Olson KE, Miller BR, Powers AM. (2004). Infection patterns of o’nyong – nyong virus in the malaria-transmitting mosquito Anopheles gambiae. Insect Mol Biol. 13:625-635.

Myles KM, Pierro DJ, Olson KE. (2004). A comparison of the transmission potential of two genetically distinct Sindbis viruses following per os infection of Aedes aegypti (Diptera: Culicidae). J. Med. Entomol. 41:95-106.

Foy BD, Myles KM, Pierro DJ, Sanchez-Vargas I, Uhlirova, M, Jindra M, Beaty BJ, Olson KE. (2004). Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species. Insect Mol. Biol. 13:89-100.

Myles KM, Pierro DJ, Olson KE. (2003). Deletions in the putative cell receptor-binding domain of Sindbis virus strain MRE16 E2 glycoprotein reduce midgut infectivity in Aedes aegypti. J. Virol. 77:8872-81.

Pierro DJ, Myles KM, Foy BD, Beaty BJ, Olson KE. (2003). Development of an orally infectious Sindbis virus transducing system that efficiently disseminates and expresses green fluorescent protein in Aedes aegypti. Insect Mol. Biol. 12:107-16.

de Lara Capurro M, Coleman J, Beerntsen BT, Myles KM, Olson KE, Rocha E, Krettli AU, James AA. (2000). Virus-expressed, recombinant single-chain antibody blocks sporozoite Invasion of salivary glands in Plasmodium gallinaceum– infected Aedes aegypti. Amer. J. Trop. Med. Hyg. 62:427-433.

Olson KE, Myles KM, Seabaugh RC, Higgs S, Carlson JO, Beaty BJ. (2000) Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection. Insect Mol. Biol. 9:57-65.

Higgs S, Oray CT, Myles K, Olson KE, Beaty BJ. (1999) Infecting larval arthropods with a Chimeric double subgenomic Sindbis virus vector to express genes of interest.Biotechniques. 27:908-911.