← All People

Keyan Zhu-Salzman

Zhu-Salzman, Keyan
Keyan Zhu-Salzman
Professor
Email:
Phone:
979.458.3357
Undergraduate Education
B.S. Fudan University, China (1985)
Graduate Education
Ph.D Purdue University (1994)
M.S. Fudan University, China (1988)

RESEARCH: Over millions of years of co-evolution with insects, plants have developed various defense machineries that can be activated in response to insect herbivory.  Insects, in turn, have developed a variety of strategies to evade these plant defense mechanisms.  An improved understanding of this complex plant defense and insect counter-defense relationship will facilitate development of better strategies to improve host plant defense.  Currently, we are using Arabidopsis to study plant defense signal transduction pathways against insect pests.  Meanwhile, since effectiveness of plant defense is also determined by the insect response, my laboratory is also investigating how insects adapt to the challenge of plant defense molecules, as well as to human imposed management strategies, and is working to identify new insect vulnerable systems.

TEACHING: I teach undergraduate courses: ENTO 428 (Insect Biotechnology) and ENTO 429 (Insect Biotechnology Laboratory

Publications

69. Zhu-Salzman, K. and R.S. Zeng (2015) Insect response to plant defensive protease inhibitors. Annu. Rev. Entomol. 60: 233-252

68. Cheng, W., J. Lei, C.W. Fox. J.S. Johnston and K. Zhu-Salzman (2015) Comparison of life history and genetic properties of cowpea bruchid strains and their response to hypoxia. J. Insect Physiol. 75: 5-11

67. Bernal, J.S., J.E. Melancon, K. Zhu-Salzman (2015) Clear advantages for fall armyworm larvae from feeding on maize relative to its ancestor Balsas teosinte may not be reflected in their mother’s host choice. Entomologia Experimentalis et Applicata 155: 206-217

66. Sang, W., L. He, X-P. Wang, K. Zhu-Salzman and C-L. Lei (2015) Evaluation of reference genes for RT-qPCR in Tribolium castaneum under UVB stress. Environ. Entomol. 44:418-425

65. Lei, J. and K. Zhu-Salzman (2015) Enhanced aphid detoxification when confronted by a host with elevated ROS production. Plant Signaling & Behavior 10: 4, e1010936

64. Wang, L., Y. H. Chi, F. Guo, H. Li-Byarlay, S. Balfe, J. Fang, B.R. Pittendrigh, K. Zhu-Salzman (2015) Transcriptomic response of cowpea bruchids to N-acetylglucosamine-specific lectins. Insect Sci. 22, 83–94

63. Lei, J., S.A. Finlayson, R.A. Salzman, L. Shan and K. Zhu-Salzman (2014) BOTRYTIS-INDUCED KINASE1 modulates Arabidopsis resistance to green peach aphids via PHYTOALEXIN DEFICIENT4. Plant Physiol. 165: 1657-1670

62. Guo, H., Y. Sun, Y. Li, P. Wang, X. Liu, K. Zhu-Salzman, and F. Ge (2014) Elevated CO2 alters the feeding behavior of the pea aphid by modifying the physical and chemical resistance of Medicago truncatula. Plant, Cell Environ. 37:2158-1568

61. Song, Y.Y., M. Ye, C. Li, X. He, K. Zhu-Salzman, R.L. Wang, Y.J. Su, S.M. Luo and R.S. Zeng (2014) Hijacking common mycorrhizal networks for herbivore-induced defence signal transfer between tomato plants. Scientific Reports 4: 3915

60. Castillo Lopez, D., K. Zhu-Salzman, M.J. Ek-Ramos and G.A. Sword (2014) The Entomopathogenic fungal endophytes Purpureocillium lilacinum (formerly Paecilomyces lilacinus) and Beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions. PLoS ONE 9: e103891

59. Yuan, Y., P.H. Krogh, X. Bai, D. Roelofs, F. Chen, K. Zhu-Salzman, Y. Liang, Y. Sun and F. Ge (2014) Microarray detection and qPCR screening of potential biomarkers of Folsomia candida (Collembola: Isotomidae) exposed to Bt proteins (Cry1Ab and Cry1Ac). Environ. Pollut. 184: 170-178

58. Guo, H., Y. Sun, Y. Li, X. Liu, Q. Ren, K. Zhu-Salzman and F. Ge (2013) Elevated CO2 modifies N acquisition of Medicago truncatula by enhancing N fixation and reducing nitrate uptake from soil. PLoS ONE 8: e81373

57. He N, Zhang C, Qi X, Zhao S, Tao Y, Yang G, Lee TH, Wang X, Cai Q, Li D, Lu M, Liao S, Luo G, He R, Tan X, Xu Y, Li T, Zhao A, Jia L, Fu Q, Zeng Q, Gao C, Ma B, Liang J, Wang X, Shang J, Song P, Wu H, Fan L, Wang Q, Shuai Q, Zhu J, Wei C, Zhu-Salzman K, Jin D, Wang J, Liu T, Yu M, Tang C, Wang Z, Dai F, Chen J, Liu Y, Zhao S, Lin T, Zhang S, Wang J, Wang J, Yang H, Yang G, Wang J, Paterson AH, Xia Q, Ji D and Xiang Z. (2013) Draft genome sequence of the mulberry tree Morus notabilis. Nat Commun. 4: 2445

56. Ye, M., Y.Y. Song, J. Long, R.L. Wang, S.R. Baerson, Z. Pan, K. Zhu-Salzman, J.F. Xie, K.Z. Cai, S.M. Luo and R.S. Zeng (2013) Priming of jasmonate-mediated anti-herbivore defense responses in rice by silicon. Proc. Natl. Acad. Sci. USA. 110: E3631-E3639

55. Guo, H., Y. Sun, B. Tong, M. Harris, K. Zhu-Salzman, and F. Ge (2013) Pea aphid promotes amino acid metabolism both in Medicago truncatula and bacteriocytes to favor aphid population growth under elevated CO2. Global Change Biol. 19: 3210–3223

54. Cheng, W., J. Lei, J-E. Ahn, Y. Wang, C.L. Lei and K. Zhu-Salzman (2013) CO2 enhances effects of hypoxia on mortality, development, and gene expression in cowpea bruchid, Callosobruchus maculatus. J. Insect Physiol. 59: 1160-1168

53. Sun, Y., H. Guo, K. Zhu-Salzman and F. Ge (2013) Elevated CO2 increases the abundance of the peach aphid on Arabidopsis by reducing jasmonic acid defenses. Plant Sci. 210: 128– 140

52. Ahn, J-E., X. Zhou, S.E. Dowd, R.S. Chapkin and K. Zhu-Salzman (2013) Insight into hypoxia tolerance in cowpea bruchid: metabolic repression and heat shock protein regulation via hypoxia-inducible factor 1. PLoS ONE 8: e57267

51. Cheng, W., J. Lei, W.L. Rooney, T.-X. Liu and K. Zhu-Salzman (2013) High basal defense gene expression determines sorghum resistance to the whorl-feeding insect southwestern corn borer. Insect Sci. 20: 307-317

50. Guo, F., J. Lei, Y. Sun, Y. H. Chi, F. Ge, B. Patil, H. Koiwa, R.S. Zeng and K. Zhu-Salzman (2012) Antagonistic regulation, yet synergistic defense: Effect of bergapten and protease inhibitor on development of cowpea bruchid, Callosobruchus maculatus. PLoS ONE 7: e41877

49. Jing, X., H. Vogel, R. Grebenok, K. Zhu-Salzman and S. Behmer (2012) Dietary sterols/steroids and the generalist caterpillar Helicoverpa zea: physiology, biochemistry and midgut gene expression. Insect Biochem. Mol. Biol. 42: 835-845

48. Wang, R.L., Y.L. Sun, X.T. Liang, Y.Y. Song, Y.J. Su, K. Zhu-Salzman and R.S. Zeng (2012) Effects of six plant secondary metabolites on activities of detoxification enzymes in Spodoptera litura. Acta Ecologica Sinica 32: 5191-5198

47. Ren, S., S. Weeda, K. Zhu-Salzman and T. Ferrell (2012) Novel Arabidopsis jasmonate-responsive mutants have variations in bacterial disease resistance. J. Biotech Res. 4: 80-91

46. Guo, H., Y. Sun, Q. Ren, K. Zhu-Salzman, L. Kang, C. Wang, C. Li and F. Ge (2012) Elevated CO2 reduces the resistance and tolerance of tomato plants to Helicoverpa armigera by suppressing the JA signaling pathway. PLoS ONE 7: e41426

45. Wu, G.Z., L. Hu, M. Ye, R.L. Wang, K. Zhu-Salzman and R.S. Zeng (2012) Effects of soybean trypsin inhibitor and defense signaling compounds on detoxification enzymes in Spodoptera litura (F.) larvae. Chinese J. Appl. Ecol. 23: 1952-1958

44. Ye, M., S.M. Luo, J.F. Xie, Y.F. Li, T. Xu, Y. Liu, Y.Y. Song, K. Zhu-Salzman and R.S. Zeng (2012) Silencing COI1 in rice increases susceptibility to chewing insects and impairs inducible defense. PLoS ONE 7: e36214

43. Cheng, W., J. Lei, J-E. Ahn, T.-X. Liu and K. Zhu-Salzman (2012) Effects of decreased O2 and elevated CO2 on survival, development, and gene expression in cowpea bruchids. J. Insect Physiol. 58: 792-800

42. Wu, G.Z., N.W. Yin, L. Hu, M. Ye, Y.Y. Song, K. Zhu-Salzman and R.S. Zeng (2012) Effects of soybean trypsin inhibitor and defense signaling compounds on the protective enzymes in Spodoptera litura larvae. Chinese J. Ecol. 31: 652-658

41. Chi, Y.H., X. Jing, J. Lei, J-E. Ahn, Y.D. Koo, D-J. Yun, S.Y. Lee, S. Behmer, H. Koiwa and K. Zhu-Salzman (2011) Stability of AtVSP in the insect digestive canal determines its defensive capability. J. Insect Physiol. 57: 391-399

40. Chi, Y.H., J-E. Ahn, D-J. Yun, S.Y. Lee, T.-X. Liu and K. Zhu-Salzman (2011) Changes in oxygen and carbon dioxide environment alter gene expression of cowpea bruchids. J. Insect Physiol. 57: 220-230

39. Ahn, J-E., L.A. Guarino and K. Zhu-Salzman (2010) Coordination of hepatocyte nuclear factor 4 and seven-up controls insect counter-defense cathepsin B expression. J. Biol. Chem. 285: 6573-6584

38. Chi, Y.H., Y.D. Koo, S.Y. Dai, J-E. Ahn, D-J. Yun, S.Y. Lee and K. Zhu-Salzman (2010) N-Glycosylation at non-canonical Asn-X-Cys sequence of recombinant cowpea bruchid cathepsin B-like counter-defense protein. Comp. Biochem. Physiol. 156B: 40-47

37. Peters, L., K. Zhu-Salzman and T. Pankiw (2010) Effect of primer pheromones and pollen diet on the food producing glands of worker honey bees (Apis mellifera L.). J. Insect Physiol. 56: 132-137

36. Ahn, J-E. and K. Zhu-Salzman (2009) CmCatD, a cathepsin D-like protease has a potential role in insect defense against a phytocystatin. J. Insect Physiol. 55: 678-685

35. Chi, Y.H., R.A. Salzman, S. Balfe, J-E. Ahn, W. Sun, J. Moon, D-J. Yun, S.Y. Lee, T.J.V. Higgins, B. Pittendrigh, L.L. Murdock and K. Zhu-Salzman (2009) Cowpea bruchid midgut transcriptome response to a soybean cystatin—costs and benefits of counter-defense. Insect Mol. Biol. 18: 97-110

34. Zhu-Salzman, K. and R.S. Zeng (2008) Molecular mechanisms of insect adaptation to plant defense – lessons learned from a bruchid beetle. Insect Sci. 15: 477-481

33. Koo, Y.D., J-E. Ahn, R.A. Salzman, J. Moon, Y.H. Chi, D-J. Yun, S.Y. Lee, H. Koiwa and K. Zhu-Salzman (2008) Functional expression of an insect cathepsin B-like counter-defense protein. Insect Mol. Biol. 17:235-245

32. Zhu-Salzman, K., D.S. Luthe and G.W. Felton (2008) Arthropod-inducible proteins: Broad spectrum defenses against multiple herbivores. Plant Physiol. 146: 852-858

31. Ahn, J-E. , L.A. Guarino and K. Zhu-Salzman (2007) Seven-up facilitates insect counter-defense by suppressing cathepsin B expression. FEBS J. 274: 2800-2814

30. Amirhusin, B., R.E. Shade, H. Koiwa, P.M. Hasegawa, R.A. Bressan, L.L. Murdock and K. Zhu-Salzman (2007) Protease inhibitors from several classes work synergistically against Callosobruchus maculatus. J. Insect Physiol. 53: 734-740

29. Ahn, J-E., M.R. Lovingshimer, R.A. Salzman, J.K. Presnail, A.L. Lu, H. Koiwa and K. Zhu-Salzman (2007) Cowpea bruchid Callosobruchus maculatus counteracts dietary protease inhibitors through modulating propeptides of major digestive enzymes. Insect Mol. Biol. 16: 295-304

28. Zhu-Salzman, K. and L.L. Murdock (2006) Cowpea: insects, ecology and control. An invited article for the Encyclopedia of Pest Management, published by Marcel Dekker.

27. Liu, Y., J-E. Ahn, S. Datta, R.A. Salzman, J. Moon, B. Huyghues-Despointes, B. Pittendrigh, L.L. Murdock, H. Koiwa and K. Zhu-Salzman (2005) Arabidopsis vegetative storage protein is an anti-insect acid phosphatase. Plant Physiol. 139: 1545-1556

26. Sagili, R.R., T. Pankiw and K. Zhu-Salzman (2005) Effects of soybean trypsin inhibitor on hypopharyngeal gland protein content, total midgut protease activity and survival of the honey bee (Apis mellifera L.). J. Insect Physiol. 51: 953-957

25. Zhu-Salzman, K., J.L. Bi and T.X. Liu (2005) Molecular strategies of plant defense and insect counter-defense. Insect Sci. 12: 3-15

24. Liu, Y., R.A. Salzman, T. Pankiw and K. Zhu-Salzman (2004) Transcriptional regulation in southern corn rootworm larvae challenged by soyacystatin N. Insect Biochem. Mol. Biol. 34: 1069-1077

23. Pankiw, T., R. Roman, R.R. Sagili, and K. Zhu-Salzman (2004) Pheromone-modulated behavioral suites influence colony growth in the honey bee (Apis mellifera). Naturwissenschaften 91: 575-578

22. Ahn, J-E., R.A. Salzman, S.C. Braunagel, H. Koiwa and K. Zhu-Salzman (2004) Functional roles of specific bruchid protease isoforms in adaptation to a soybean protease inhibitor. Insect Mol. Biol. 13: 649-657

21. Moon, J., R.A. Salzman, J-E, Ahn, H. Koiwa and K. Zhu-Salzman (2004) Transcriptional regulation in cowpea bruchid guts during adaptation to a plant defense protease inhibitor. Insect Mol. Biol. 13: 283-291

20. Amirhusin, B., R.E. Shade, H. Koiwa, P.M. Hasegawa, R.A. Bressan, L.L. Murdock and K. Zhu-Salzman (2004) Soyacystatin N inhibits proteolysis of wheat -amylase inhibitor and potentiates toxicity against cowpea bruchid, Callosobruchus maculatus. J. Econ. Entomol. 97: 2095-2100

19. Zhu-Salzman, K., R.A. Salzman, J-E. Ahn and H. Koiwa (2004) Transcriptional regulation of sorghum defense determinants against a phloem-feeding aphid. Plant Physiol. 134: 420-431

18. Zhu-Salzman, K., H. Li , P.E. Klein and R.L. Gorena (2003) Using high-throughput amplified fragment length polymorphism to distinguish sorghum greenbug (Homoptera: Aphididae) biotypes. Agr. Forest Entomol. 5: 311-315

17. Zhu-Salzman, K., H. Koiwa, R.A. Salzman, R.E. Shade and J-E. Ahn (2003) Cowpea bruchid Callosobruchus maculatus uses a three-component strategy to overcome a plant defensive cysteine protease inhibitor. Insect Mol. Biol. 12: 135-145

16. Zhu-Salzman, K., J-E. Ahn, R.A. Salzman, H. Koiwa, R.E. Shade and S. Balfe (2003) Fusion of a soybean cysteine protease inhibitor and a legume lectin enhances anti-insect activity synergistically. Agr. Forest Entomol. 5: 317-323

15. Zhu-Salzman, K., P.K. Hammen, R.A. Salzman, H. Koiwa, R.A. Bressan, L.L. Murdock and P.M. Hasegawa (2002) Calcium modulates protease resistance and carbohydrate binding of a plant defense legume lectin, Griffonia simplicifolia lectin II (GSII). Comp. Biochem. Physiol. 132B: 327-334

14. Zhu-Salzman, K. (2002) Book review for “Recombinant Protease Inhibitors in Plants”. Ann. Entomol. Soc. Am. 95: 651

13. Zhu-Salzman, K. and R.A. Salzman (2001) Functional mechanics of the plant defensive Griffonia simplicifolia lectin II: resistance to proteolysis is independent of glycoconjugate binding in the insect gut. J. Econ. Entomol. 94: 1280-1284

12. Koiwa, H., M.P. D’Urzo, I. Assfalg-Machleidt, K. Zhu-Salzman, R.E. Shade, H. An, L.L. Murdock, W. Machleidt, R.A. Bressan and P.M. Hasegawa (2001) Phage display selection of hairpin loop soyacystatin variants that mediate high affinity inhibition of a cysteine proteinase. Plant J. 27: 383-391

11. Koiwa, H., M.P. D’Urzo, K. Zhu-Salzman, J.I. Ibeas, R.E. Shade, L.L. Murdock, R.A. Bressan, P.M. Hasegawa (2000) An in-gel assay of a recombinant western corn rootworm (Diabrotica virgifera virgifera) cysteine proteinase expressed in yeast. Analytical Biochem. 282:153-155

10. Koiwa, H., R.E. Shade, K. Zhu-Salzman, M.P. D’Urzo, L.L. Murdock, R.A. Bressan and P.M. Hasegawa (2000) A plant defensive cystatin (soyacystatin) targets cathepsin L-like digestive cysteine proteinases (DvCALs) in the larval midgut of western corn rootworm (Diabrotica virgifera virgifera). FEBS Lett. 471:67-70

9. Salzman, R.A., T. Fujita, K. Zhu-Salzman, P.M. Hasegawa and R.A. Bressan (1999) An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. Plant Mol. Biol. Rep. 17:11-17

8. Zhu-Salzman, K., R.E. Shade, H. Koiwa, R.A. Salzman, M. Narasimhan, R.A. Bressan, P.M. Hasegawa and L.L. Murdock (1998) Carbohydrate-binding and resistance to proteolysis control insecticidal activity of Griffonia simplicifolia lectin II (GS-II). Proc. Natl. Acad. Sci. USA 95: 15123-15128

7. Zhu-Salzman, K., R.A. Salzman, H. Koiwa, L.L. Murdock, R.A. Bressan and P.M. Hasegawa (1998) Ethylene negatively regulates local expression of plant defense lectin genes. Physiol. Plant. 104: 365-372

6. Koiwa, H., R.E. Shade, K. Zhu-Salzman, L. Subramanian, L.L. Murdock, S.S. Nielsen, R.A. Bressan and P.M. Hasegawa (1998) Phage display selection can differentiate insecticidal activity of soybean cystatins. Plant J. 14: 371-379

5. Koiwa, H., L. Subramanian, R.E. Shade, K. Zhu-Salzman, S.S. Nielsen, L.L. Murdock, R.A. Bressan and P.M. Hasegawa (1997) Insecticidal activities of soybean cysteine proteinase inhibitors are correlated with their papain-binding affinities as phage-displayed proteins. Plant Physiol. 114: S-220

4. Zhu-Salzman, K., R.A. Bressan, P.M. Hasegawa and L.L. Murdock (1996) Identification of N-acetylglucosamine binding residues in Griffonia simplicifolia lectin II. FEBS Lett. 390: 271-274

3. Zhu-Salzman, K., J.E. Huesing, R.E. Shade, R.A. Bressan, P.M. Hasegawa and L.L. Murdock (1996) An insecticidal N-acetylglucosamine-specific lectin gene from Griffonia simplicifolia (Leguminosae). Plant Physiol. 110: 195-202

2. Zhu-Salzman, K., L. Ke and J. Xin (1996) Selection and genetic analysis of fenvalerate-resistance in Amblyseius pseudolongispinosus (Acari: Phytoseiidae). Systematic Applied Acarol. 1: 5-10

1. Zhu-Salzman, K., J.E. Huesing, R.E. Shade and L.L. Murdock (1994) Cowpea trypsin inhibitor and resistance to cowpea weevil (Coleoptera: Bruchidae) in cowpea variety ‘TVu 2027’. Environ. Entomol. 23: 987-991

Book Chapters:

3. Zhu-Salzman, K. and T.X. Liu (2011) Insect herbivory-inducible proteins confer post-ingestive plant defenses. In Recent Advances in Entomological Research: From Molecular Biology to Pest Management. pp. 500. T.X. Liu and L. Kang (Eds.), High Education Press (Beijing) and Springer (Heidelberg).

2. Zhu-Salzman, K. (2008) Insect adaptation to plant defense: Decryption of gut protease deployment. In Recent advances in Insect Physiology, Toxicology and Molecular Biology. Nannan Liu (Ed.), Research Signpost, pp 199-208.

1. Zhu-Salzman, K. and T.X. Liu (2005) Co-evolutionary arms race – Plant defense and insect Counter-defense. In Entomological Research: Progress and Perspectives. T.X. Liu and L. Kang (Eds.), Science Press, Beijing, pp31-44.